Horn was born in Ottawa, and grew up both in Ottawa and on the Kahnawake Mohawk reserve outside of Montreal. Her mother, Kahn-Tineta Horn, is a Mohawk former model and a political activist for the Kahnawake First Nation. Her father, who is of German and Scottish descent, is a lawyer. Horn, her mother, and her older sister Waneek (later a broadcaster and co-captain of the Canadian women's water polo team at the 2000 Sydney Olympics) were notable participants in the 1990 Oka Crisis. Waneek was stabbed in the chest by a soldier wielding a bayonet while holding Horn, who was then aged four; a photograph of the incident, published on the front page of newspapers, symbolized the standoff between Mohawks and the Canadian government.
Horn decided to be an actress at a young age, but concentrated on swimming and water polo as a teenager. She graduated from Dawson College in 2005 for theatre arts, and appeared in a number of short films.
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